Protein is an important source of drug development and industrial enzyme preparation. It is important to reduce side effects and improve the physical and biological properties of engineered proteins. Glycosylation is a common modification of proteins. The process of changing the glycosylation to alter the properties of proteins is known as glycoengineering. The importance of glycoengineering in overcoming the limitations of proteins has been established by research in many disciplines.
As the field of high-throughput screening technology and biochemistry continues to update, in vitro glyco engineering is becoming increasingly important due to its rapid development speed and low time and other costs. Profacgen uses IVGE techniques to optimize some necessary glycotransferases (such as sialic acid transferase and galactosyltransferase) for the drug development phase by modifying glycosidic bonds or enriching certain sugar types (green in Figure 2) using discrete enzyme reactions with clear kinetics and predictable results. IVGE can modify glycosylation in a controlled manner after proteins are produced and purified, and thus can isolate glycosylation management from cell culture manipulations.